Polar Destabilization of DNA Duplexes with Single-Stranded Overhangs by the Deinococcus radiodurans SSB Protein†
Identifieur interne : 002D67 ( Main/Exploration ); précédent : 002D66; suivant : 002D68Polar Destabilization of DNA Duplexes with Single-Stranded Overhangs by the Deinococcus radiodurans SSB Protein†
Auteurs : Julie M. Eggington [États-Unis] ; Alexander G. Kozlov [États-Unis] ; Michael M. Cox [États-Unis] ; Timothy M. Lohman [États-Unis]Source :
- Biochemistry [ 0006-2960 ] ; 2006.
Abstract
The Deinococcus radiodurans SSB protein has an occluded site size of 50 ± 2 nucleotides on ssDNA but can form a stable complex with a 26−30-nucleotide oligodeoxynucleotide using a subset of its four ssDNA binding domains. Quantitative estimates of D. radiodurans SSB protein in the D. radiodurans cell indicate approximately 2500−3000 dimers/cell, independent of the level of irradiation. At biologically relevant concentrations, when bound at single-strand−double-strand DNA junctions in vitro, D. radiodurans SSB protein has a limited capacity to displace the shorter strand of the duplex, permitting it to bind to single-strand extensions shorter than 26−30 nucleotides. The capacity to displace the shorter strand of the duplex shows a pronounced bias for extensions with a free 3‘ end. The Escherichia coli SSB protein has a similar but somewhat less robust capacity to displace a DNA strand annealed adjacent to a single-strand extension. These activities are likely to be relevant to the action of bacterial SSB proteins in double-strand break repair, acting at the frayed ends created by ionizing radiation.
Url:
DOI: 10.1021/bi061178m
Affiliations:
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by the <hi rend="italic">Deinococcus radiodurans</hi> SSB Protein<ref type="bib" target="#bi061178mAF2">†</ref></title><author><name sortKey="Eggington, Julie M" sort="Eggington, Julie M" uniqKey="Eggington J" first="Julie M." last="Eggington">Julie M. Eggington</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName><wicri:cityArea>Department of Biochemistry, University of Wisconsin−Madison, 433 Babcock Drive, Madison,Wisconsin 53706-1544, and Department of Biochemistry and Molecular Biophysics,Washington University School of Medicine, 660 South Euclid Avenue, St. Louis</wicri:cityArea></affiliation><affiliation></affiliation></author><author><name sortKey="Kozlov, Alexander G" sort="Kozlov, Alexander G" uniqKey="Kozlov A" first="Alexander G." last="Kozlov">Alexander G. 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Cox</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName><wicri:cityArea>Department of Biochemistry, University of Wisconsin−Madison, 433 Babcock Drive, Madison,Wisconsin 53706-1544, and Department of Biochemistry and Molecular Biophysics,Washington University School of Medicine, 660 South Euclid Avenue, St. Louis</wicri:cityArea></affiliation><affiliation></affiliation><affiliation></affiliation></author><author><name sortKey="Lohman, Timothy M" sort="Lohman, Timothy M" uniqKey="Lohman T" first="Timothy M." last="Lohman">Timothy M. 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Quantitative estimates of D. radiodurans SSB protein in the D. radiodurans cell indicate approximately 2500−3000 dimers/cell, independent of the level of irradiation. At biologically relevant concentrations, when bound at single-strand−double-strand DNA junctions in vitro, D. radiodurans SSB protein has a limited capacity to displace the shorter strand of the duplex, permitting it to bind to single-strand extensions shorter than 26−30 nucleotides. The capacity to displace the shorter strand of the duplex shows a pronounced bias for extensions with a free 3‘ end. The Escherichia coli SSB protein has a similar but somewhat less robust capacity to displace a DNA strand annealed adjacent to a single-strand extension. These activities are likely to be relevant to the action of bacterial SSB proteins in double-strand break repair, acting at the frayed ends created by ionizing radiation.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Missouri (État)</li></region></list><tree><country name="États-Unis"><region name="Missouri (État)"><name sortKey="Eggington, Julie M" sort="Eggington, Julie M" uniqKey="Eggington J" first="Julie M." last="Eggington">Julie M. Eggington</name></region><name sortKey="Cox, Michael M" sort="Cox, Michael M" uniqKey="Cox M" first="Michael M." last="Cox">Michael M. Cox</name><name sortKey="Kozlov, Alexander G" sort="Kozlov, Alexander G" uniqKey="Kozlov A" first="Alexander G." last="Kozlov">Alexander G. Kozlov</name><name sortKey="Lohman, Timothy M" sort="Lohman, Timothy M" uniqKey="Lohman T" first="Timothy M." last="Lohman">Timothy M. Lohman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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